Table I. Wound-regulated proteins in phloem latex identified by isotope-coded protein labeling

MW, Molecular mass in kD.

AccessionaMWW3/CbW24/CbDescription
Carbohydrate and energy metabolism
 42916231.12.212.4914-3-3 protein 32-kD endonuclease
 Cucsa.08998035.90.720.58GDP-l-Fuc synthase
 Cucsa.30267033.60.330.33UDP-l-Rha synthase
 Csa02248259.20.330.43Glc-6-P dehydrogenase
 Cucsa.17012040.90.590.62SNF1-related protein kinase2
 174300957.80.090.09SNF1-related protein kinase1
Signaling, defense
 1099833648.13.254.06Silverleaf whitefly-induced protein1
 11304596017.91.091.69MAPK6
 31943958533.80.791.70Cyclin-dependent kinase A
 11074860842.71.071.63NO3 stress-induced MAPK
 11757366416.51.824.1316-kD phloem protein1, PP16-1
 175309995.31.062.09Phloem filament protein, PP1
 50844524.50.471.09Dimeric phloem-specific lectin, PP2
 9990699735.60.591.15Class III peroxidase precursor
 2045301364.00.610.90Phloem calmodulin-like domain protein kinase
 Cucsa.14250016.90.830.56Calmodulin
 502622137.60.501.09Putative chymotrypsin protease inhibitor
Chaperones
 Csa00169779.92.242.67HSP81
 Cucsa.14261060.51.782.27Chaperonin-containing complex protein1
 Csa00963459.01.432.00T-complex protein1 subunit ζ-like
 6272858718.11.081.73Cyclophilin
 Cucsa.10659013.20.960.59Peptidyl-prolyl cis-trans-isomerase Pin1
 5147739463.80.571.21Protein disulfide isomerase (PDI)-like protein3
Protein synthesis and degradation
 Cucsa.15323067.13.231.38DEAD box RNA helicase
 Cucsa.16242053.51.421.72DEAD box RNA helicase
 Cucsa.34511085.12.042.42Elongation factor2-like
 Cucsa.20194016.62.401.66Ubiquitin-conjugating enzyme E2
 Cucsa.18180048.02.112.6426S protease subunit 6A-like
 8107630716.51.632.00Putative translation initiation factor eIF-1A-like
 22411024417.50.981.81Proteasome subunit α type 2
 22408568826.61.051.79Proteasome subunit α type 1
 Csa01170986.71.581.77Cullin-1
 14780008566.70.531.26E3 ubiquitin ligase ARI7-like
 22409556197.60.581.1326S proteasome regulatory complex component
 Cucsa.01814051.20.600.78Eukaryotic translation initiation factor3 subunit 6N
 Cucsa.34222045.30.640.48COP9 signalosome subunit4-like
 30713642925.50.410.34COP9 signalosome subunit7a
 29835299717.20.310.36Putative ubiquitin-conjugating enzyme
Transport, cytoskeleton
 49573123.02.381.79Small ras-related
 609786913.80.933.52Actin
 Csa01039041.71.711.26Actin
 Cucsa.05358017.12.181.59Actin-depolymerizing factor
 15746721918.52.441.73GTP-binding nuclear protein Ran3-like
 30713595749.50.611.81Tubulin α-chain
 12319243125.31.431.89Ran1
 13746068.80.831.73V-type proton ATPase catalytic subunit A
 25557059996.40.531.01Importin β-1, putative
 5147737999.80.521.26DRP, dynamin
Others
 Cucsa.32844043.21.531.71S-Adenosyl-Met synthetase
 30713593432.91.701.06Pyridoxal biosynthesis protein
 16804952589.60.551.18Cell division control protein48, CDC48
  • a Accession numbers from the National Center for Biotechnology Information, International Cucurbit Genomics Initiative (Csa identifiers), and the Joint Genome Institute (Cucsa identifiers).  bC, W3, W24 indicate phloem latex from untreated control plants or wounded plants at 3- and 24-h time points.