Table I. Kinetic parameters of UGT73C11 and UGT73C13 toward oleanolic acid and hederagenin
UGTSapogeninKmkcatkcat/KmKiVmax
µms−1s−1 µm−1µmnmol min−1 mg−1
UGT73C11Oleanolic acid9.7 ± 2.20.8160.084817 ± 118
Hederagenin3.3 ± 0.80.3890.118390 ± 38
UGT73C13Oleanolic acida12.5 ± 2.10.2310.019262231 ± 21
Oleanolic acidb7.6 ± 1.20.1760.023176 ± 7
Hederagenin22.9 ± 4.80.1310.006131 ± 10
  • a Kinetic parameters based on fit to the substrate inhibition equation.

  • b Kinetic parameters based on fit to the Michaelis-Menten equation.