Table II. Fit parameters describing the decay of “out-of-phase” ESE

Fit parameters are shown describing the decay of the ESE after the actinic laser flash in thylakoids isolated from the wild type and FN68 strain. Ascorbate (10 mm) with 200 µm phenazinemethosulfate as mediator was used to reduce P700+ in the experiments performed at 265 K. The other samples were dark adapted for 30 min in the presence of Na2S2O4 (11 mm, pH 8; FA/B reduced, FX oxidized). Preillumination of the sample for 5 min at 205 K results in full reduction of FA/B and more than 80% reduction of FX (FA/B/X reduced). The decays were fitted to a sum of an exponential function, y(t) = α1et/τ1 + α2et/τ2 and τav = α1 × τ1 + α2 × τ2, with α1 + α2 = 1 (where α is the associated amplitude).

Sampleτ1α1τ2α2τav
nsnsns
Decay of the ESE at 265 K
 Wild Type305 ± 40.93 ± 0.11,585 ± 480.07 ± 0.05488 ± 5
 FN68226 ± 80.37 ± 0.5964 ± 100.63 ± 0.8693 ± 7
Decay of the ESE at 100 K, FA/B reduced, FX oxidized
 Wild type2.36 ± 0.40.11 ± 0. 0822.2 ± 0.80.89 ± 0.0820.0 ± 0.9
 FN682.14 ± 0.60.09 ± 0.119.5 ± 0.40.91 ± 0.0717.9 ± 0.7
Decay of the ESE at 100 K, FA/B/X reduced
 Wild type2.13 ± 0.50.37 ± 0.0618.9 ± 0.60.63 ± 0.0212.7 ± 0.4
 FN682.05 ± 0.50.29 ± 0.0815.5 ± 0.80.71 ± 0.0711.6 ± 0.6