Table I. Global fit parameters describing the kinetics of A1 oxidation and P700+ reduction at room temperature in vivo

Global fitting analysis is shown for the optical transient in whole cells of Fud7-P71 and the FN68 mutant. The data have been fitted by the linear sum of exponential functions: Embedded Image, where Embedded Image is a nondecaying component in the time range investigated. The fractional amplitude of the components in the nanosecond range [Embedded Image] at three characteristic wavelengths is calculated as: Embedded Image.

SampleLifetimef(A390)f(A430)f(A480)
ns
Fud7-P71 (control)
 τ128.30.410.090.24
 τ2286.40.580.060.26
 τ367540.010.850.51
FN68
 τ128.40.260.040.14
 τ2312.50.610.130.28
 τ365470.130.830.54