Table V. Net fluxes of pathway intermediates determined through metabolic flux analysis in developing soybean embryos provided three different C:N ratios

Flux value rates are shown in units of micromoles per milligram dry weight produced. The 95% CIs are shown in brackets. A complete list of all fluxes including exchange fluxes is given in Supplemental Data Set S6.

Reaction Description13:121:137:1
Flux Value95% CIFlux Value95% CIFlux Value95% CI
Phosphoenolpyruvate carboxylase/carboxykinase0.86[0.75, 0.96]1.12[1.02, 1.19]0.91[0.80, 1.00]
Mitochondrial malic enzyme0.56[0.50, 0.62]0.55[0.50, 0.61]0.37[0.32, 0.42]
Plastidic malic enzyme1.06[1.01, 1.11]1.06[1.02,1.10]0.87[0.81, 0.92]
Cytosolic pyruvate kinase2.04[1.95, 2.15]1.97[1.89, 2.05]1.77[1.71, 1.86]
Plastidic pyruvate kinase2.65[2.54, 2.75]2.69[2.60, 2.79]2.97[2.86, 3.08]
Glu production2.11[2.02, 2.20]1.33[1.30, 1.67]0.80[0.77, 0.88]
Glu dehydrogenase3.51[3.35, 3.67]2.17[2.11, 2.54]1.33[1.28, 1.42]
Citrate synthase2.35[2.22, 2.48]2.24[2.14, 2.35]1.93[1.86, 2.01]
ATP-citrate lyase1.74[1.67, 1.81]1.66[1.58, 1.73]1.36[1.29, 1.42]
Isocitrate dehydrogenase0.57[0.44, 0.70]0.49[0.43, 0.58]0.52[0.47, 0.57]
2-Oxoglutarate dehydrogenase1.98[1.78, 2.16]1.33[1.25, 1.45]1.05[0.98, 1.12]
Fumarase2.01[1.85, 2.18]1.42[1.35, 1.52]1.11[1.05, 1.17]
Thr aldolase0.03[0.02, 0.04]0.02[0.01, 0.03]0.04[0.03, 0.05]
Asparaginase/Asn aminotransferase0.01[−0.04, 0.06]0.17[0.09, 0.21]0.24[0.20, 0.28]
Thr biosynthesis0.34[0.33, 0.36]0.30[0.28, 0.31]0.24[0.23, 0.25]
Carbon dioxide production10.76[9.60, 11.94]8.84[8.12, 9.69]8.37[7.48, 9.38]
Isocitrate lyase0.03[0.00, 0.08]0.10[0.07, 0.12]0.06[0.04, 0.08]
Glyoxylate-Gly transaminase0.01[−0.08, 0.03]0.01[−0.06, 0.02]0.00[−0.09, 0.01]
Malate synthase0.02[0.00, 0.12]0.09[0.06, 0.15]0.05[0.03, 0.14]