Table I. Provision of varied C:N molar ratios in culture medium

Growth and total biomass compositions were determined from soybean ‘Jack’ embryos grown on medium supplied with various concentrations of Suc, Glc, Gln, and Asn. An example calculation of the resulting C:N ratio, taking into account carbon coming from supplied amino acids, is shown in Figure 2. Culture compositions in boldface were chosen for 13C-metabolic flux analysis. Other culture parameters are described in “Materials and Methods.”

C:N RatioCarbon Supplied as Amino AcidsSubstrate Provided
SucGlcGlnAsn
%mm
640904514050
1318140708312
1318140707025
1813140705018
1913150755018
2111150754516
337170853012
376170852512
6042001001610
91318090114