Table I. Provision of varied C:N molar ratios in culture medium

Growth and total biomass compositions were determined from soybean ‘Jack’ embryos grown on medium supplied with various concentrations of Suc, Glc, Gln, and Asn. An example calculation of the resulting C:N ratio, taking into account carbon coming from supplied amino acids, is shown in Figure 2. Culture compositions in boldface were chosen for ¹³C-metabolic flux analysis. Other culture parameters are described in “Materials and Methods.”