Table II. Km and kcat values for MtNFH1, AtChiC, and NtChiV with NFs as substrates
SubstrateMtNFH1AtChiCNtChiV
KmkcatkcatKm−1KmkcatkcatKm−1KmkcatkcatKm−1
μms−1mm−1 s−1μms−1mm−1 s−1μms−1mm−1 s−1
NodSm-V(C16:2, S)52.7 ± 2.3a8.4 ± 0.6159.3 ± 17.680 ± 6.30.033 ± 0.0010.41 ± 0.0268 ± 6.40.088 ± 0.0041.3 ± 0.2
NodSm-IV(C16:2, S)60.1 ± 4.77.9 ± 0.2132.5 ± 13.4NDbNDNDNDNDND
NodSm-IV(C16:2, Ac, S)97.3 ± 12.23.5 ± 0.136.4 ± 5.5NDNDNDNDNDND
  • a Data indicate means ± sd from enzyme tests (37°C) with three independently purified protein preparations.  bND, No activity detected.