Table I. RG-II-B dimer formation and B concentration of Arabidopsis roots grown under low-B and normal-B conditions

Plants were grown on solid media containing 0.1 and 30 µm boric acid for 10 to 14 d. Cell wall fraction was prepared from plant roots and then treated with EPG. The solution of EPG-soluble material was fractionated by size-exclusion chromatography equipped with a refractive index detector. Relative proportions of RG-II-B dimer to total RG-II and B concentration of whole roots are shown (means ± sd, n = 3–4). Different superscript letters show significant difference (P < 0.05, Tukey’s test).

Plant lineB Concentration in RootsRelative Proportion of RG-II-B Dimer
0.1 µmB Concentration in Medium30 µmB Concentration in Medium0.1 µmB Concentration in Medium30 µmB Concentration in Medium
mmol kg–1 dry wt%
Col-00.39 ± 0.03a1.44 ± 0.1754.0 ± 1.1a90.1 ± 4.1
bor1-30.33 ± 0.01ab1.54 ± 0.0452.8 ± 0.4a90.0 ± 1.1
bor2-10.36 ± 0.04ab1.28 ± 0.1542.5 ± 0.5b91.6 ± 2.1
bor2-20.37 ± 0.02ab1.53 ± 0.0545.7 ± 1.0b92.0 ± 2.3
bor1-3/bor2-10.31 ± 0.03b1.43 ± 0.1245.1 ± 0.9b90.4 ± 1.5