Table I. Cell size and chlorophyll content of strains

Numbers of cells per unit of volume in cellular suspension at O.D. (750 nm) = 0.5 were measured by counting the cells directly using a Beckman Coulter 2Z particle counter. Cell diameter was directly measured using the same instrument. Cell volume was calculated from these measurements. Chlorophyll content was derived from the 750- and 680-nm values as described in “Materials and Methods.” Results are from four biological replicates. sd is indicated. Asterisks indicate significant differences between samples (P < 0.05).

StrainCells per 1 mL of Culture at O.D. (750 nm) = 1 (106 Cells)Cell DiameterCell VolumeChlorophyll ContentChlorophyll Content per Cellular Volume
µmµm3amol cell−1amol µm−3
Wild type145.2 ± 5.62.02 ± 0.054.34 ± 0.3242.1 ± 2.69.69 ± 0.20
pcpcT→C192.5 ± 13.9*1.91 ± 0.04*3.66 ± 0.25*30.5 ± 1.1*8.35 ± 0.51*
ΔCpcC1C2:pcpcT→C139.7 ± 7.21.99 ± 0.024.11 ± 0.1335.0 ± 1.3*8.52 ± 0.17*
Olive224.8 ± 4.5*1.82 ± 0.01*3.17 ± 0.06*26.4 ± 0.6*8.33 ± 0.19*