Table II. Targeted MS treatment conditions

Treatment conditions were used for targeted proteomic analysis of 58 phosphopeptides.

TreatmentConcentrationRationale
Mannitol300 mmOsmotic stress, validation of discovery results
NaCl150 mmIonic stress, osmotic stress
KCl150 mmPlasma membrane depolarization, osmotic stress
S-ABA50 μmDrought stress hormone
Cold0°C–4°CFreezing tolerance overlap with dehydration stress; overlapping second messengers with osmotic stress
Flg221 μmPathogen elicitor; overlapping second messengers with osmotic stress
H2O25 mmROS, second messenger
Fusicoccin5 μmPlasma membrane hyperpolarization
Methyl jasmonate50 μmDefense response hormone; minimal overlap expected