Table I. Flux in the MEP pathway of DXS transgenic and mutant lines

The total labeled fraction of DXP at various labeling times (Fig. 4B) was plotted versus labeling time and the data fitted to an equation describing exponential rise to maximum (A × [1 − e{−k × t}]), where A is the maximum labeling plateau, t is the labeling time, and k is the kinetic rate constant. Details are provided in the “Materials and Methods.” Flux was calculated by multiplying DXP pool size (obtained from LC-MS/MS measurements) by the fitted rate constant k.

Plant LineADXP Pool SizekFlux
pmol mg−1 dry wtmin−1pmol mg−1 dry wt min−1
dxs30.588 ± 0.0223.1 ± 1.240.1172.72 ± 0.15
Wild type0.523 ± 0.0126.9 ± 1.910.1283.44 ± 0.24
DXS120.505 ± 0.0234.3 ± 1.530.1354.64 ± 0.21
DXS160.479 ± 0.0134.3 ± 1.470.1816.20 ± 0.27
DXS240.465 ± 0.0134.5 ± 2.550.1936.68 ± 0.49
DXS450.466 ± 0.0135.2 ± 1.450.1816.36 ± 0.26