Table I. Global joint fitting of the VSD mutants of KAT1 expressed in X. laevis oocytes

Fittings were to a Boltzmann function of the form shown in Equation 1. Listed here, and shown in Figures 2 to 6, are the results of the best fitting obtained with δ held in common between all data sets and gmax held in common between all sets apart from those of the KAT1D105E, KAT1D105N, and KAT1R177K mutants. Fitted δ, 1.87 ± 0.05.

ConstructV1/2gmax
mVmS
KAT1wt−138.9 ± 0.6
KAT1F102W−225.1 ± 0.8
KAT1F102Q−217.0 ± 1.1
KAT1F102S−195.5 ± 0.983.4 ± 0.6
KAT1F102L−157.2 ± 0.5
KAT1D95E−110.3 ± 0.8
KAT1D95N−78.2 ± 0.4
KAT1D141E−141.2 ± 0.9
KAT1D105E−90.5 ± 1.259.1 ± 0.5
KAT1D105N−78.9 ± 0.751.6 ± 0.4
KAT1R177K−85.1 ± 2.144.0 ± 0.6