Table II. Pigment contents in wild-type and orrm6 mutant plants complemented with ORRM6 or NEpsbF

Chlorophyll was extracted and determined as described by Wellburn (1994). Data are presented as means ± se (n = 4–5 for pigment contents). Values not connected by the same letter are significantly different (Student’s t test, P < 0.05).

ParameterWild Type/Empty Vectororrm6-1/Empty Vectororrm6-2/Empty VectorWild Type/NEpsbForrm6-1/NEpsbForrm6-2/NEpsbFWild Type/ORRM6orrm6-1/ORRM6orrm6-2/ORRM6
Chlorophyll a (mg g−1 fresh weight)0.996 ± 0.055A0.863 ± 0.015C0.882 ± 0.017BC1.015 ± 0.025A0.974 ± 0.035AB0.954 ± 0.021ABC1.046 ± 0.050A1.027 ± 0.045A1.055 ± 0.023A
Chlorophyll b (mg g−1 fresh weight)0.258 ± 0.013ABC0.239 ± 0.004C0.256 ± 0.005ABC0.242 ± 0.007BC0.268 ± 0.007AB0.270 ± 0.005A0.261 ± 0.011ABC0.251 ± 0.012ABC0.262 ± 0.006ABC
Total chlorophyll (mg g−1 fresh weight)1.254 ± 0.068A1.102 ± 0.019C1.137 ± 0.021BC1.257 ± 0.031A1.242 ± 0.041A1.224 ± 0.025ABC1.307 ± 0.036A1.278 ± 0.057A1.316 ± 0.029A
Chlorophyll a/b3.856 ± 0.040C3.617 ± 0.010D3.449 ± 0.025E4.203 ± 0.049A3.640 ± 0.068D3.534 ± 0.042DE4.002 ± 0.050B4.101 ± 0.039AB4.033 ± 0.047B