Table IV. Transmission of amiRNA cassettes targeting EXO70C1 or EXO70C2
PlantamiRNABackground GenotypePlants Bearing amiRNA Cassette in the OffspringnComments
xC2exo70C150%Expected for a functional construct
xC2exo70C175%Expected for a nonfunctional construct
#1xC2exo70C153%128PCR genotyping
#1xC2exo70C152%195Basta selection
#2xC2exo70C144%116Basta selection
#3xC2exo70C156%81Basta selection
xC2Wild type60%aExpected for a functional construct
xC2Wild type75%Expected for a nonfunctional construct
#7xC2Wild type63%230Basta selection
#8xC2Wild type65%249Basta selection
xC1exo70C250%Expected for a functional construct
xC1exo70C275%Expected for a nonfunctional construct
#4xC1exo70C249%174Basta selection
#5xC1exo70C252%107Basta selection
#6xC1exo70C252%109Basta selection
xC1Wild type75%Expected for a functional construct
xC1Wild type75%Expected for a nonfunctional construct
#9xC1Wild type79%100Basta selection
#10xC1Wild type76%382Basta selection
  • a Estimated based on the transmission efficiency of the exo70C2-1 allele.