Table II. Enzyme kinetic parameters for wild-type SbCAD2, SbCAD4, and SbCAD4 mutants
Kinetic ParametersWild-Type SbCAD2Wild-Type SbCAD4W58LY95VL119WA278VY288PG301FL119W/G301F
p-Coumaraldehyde
 kcat (s−1)11.932.173.273.591.083.031.199.5030.16
 Kmm)69.754,633.237,101.666,882.542,115.017,966.014,178.84267.9880.88
 Kim)32,672.9220,585.1510,021.797,633.014.3E+12331,916.867.5E+11398.93160.03
 kcat/Km (s−1 μm−1)0.174.7E-044.6E-045.2E-045.1E-043.8E-042.8E-040.040.37
Coniferaldehyde
 kcat (s−1)12.7720.283.689.262.330.763.7671.5725.83
 Kmm)95.18233.209,173.7267.9610.3821.38386.735,679.2928.01
 Kim)122.62295.1212.07427.204,171.926,030.88710.8916.2011,472.15
 kcat/Km (s−1 μm−1)0.130.094.0E-040.140.220.040.010.010.92
Sinapaldehyde
 kcat (s−1)31.7512.542.5039.973.321.051.595.2918.98
 Kmm)59.7633.345,522.36111.9578.3179.317.8216.83105.19
 Kim)83.01111.751,455.7437.391,056.372,912.791,391.651,232.6768,075.58
 kcat/Km (s−1 μm−1)0.530.384.5E-040.360.040.010.200.310.18