Table I. Kinetic parameters (Vmax, Km, and Hill coefficient) of purified BAM1, BAM2, BAM3, and BAM5 from Arabidopsis

The substrate was soluble starch. Values are means ± sd from three replicate data sets. Within each parameter, means that are not significantly different (P > 0.05) share the same letter (Student’s t test).

EnzymeVmaxKmHill Coefficient
nmol min−1 mg−1 proteinmg mL−1 soluble starch
BAM1892 ± 59 a11.9 ± 2.9 a0.91 ± 0.09 a
BAM2393 ± 52 b45.8 ± 2.8 b3.11 ± 0.21 c
BAM31,119 ± 91 c26.3 ± 8.8 c1.13 ± 0.12 b
BAM5207 ± 23 d2.3 ± 0.1 d1.32 ± 0.07 a,b