Table 1. Complementation of a sim mutant by transgenic constructs with mutations in various motifs of SIM

The multicellular trichome phenotype of a complementation line homozygous for a single T-DNA insert for each of the indicated SMRs was assessed by counting the number of 4,6-diamidino-2-phenylindole (DAPI)-stained nuclei at each trichome initiation site (TIS) for each genotype. All values followed by the letter a have significantly fewer nuclei per TIS than the sim mutant (P < 1 × 10−11 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). For each transgenic genotype, at least two additional independent homozygous T3 lines were obtained having a phenotype qualitatively equivalent to the line shown here.

GenotypeNo. of Nuclei per TISNo. of TIS
Col-01.00 ± 0.00 a50
Sim2.16 ± 0.9850
sim GL2pro:YFP:SIM1.03 ± 0.18 a50
sim GL2pro:YFP:motifA1.98 ± 0.8050
sim GL2pro:YFP:motifB1.96 ± 0.6750
sim GL2pro:YFP:motifC1.02 ± 0.14 a50