Table 3. Redundant NLSs in the C-terminal end of SIM are required for its full function

The multicellular trichome phenotype of a complementation line homozygous for a single T-DNA insert for each of the indicated SMRs was assessed by counting the number of DAPI-stained nuclei at each trichome initiation site (TIS) for each genotype. All genotypes followed by the letter a have significantly fewer nuclei per TIS than the sim mutant (P < 1 × 10−9 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). The genotype followed by the letter b has significantly fewer nuclei than the sim mutant (P < 0.01 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests) but significantly more nuclei per TIS than Col-0 (P < 1 × 10−6 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). For each transgenic genotype, at least two additional independent homozygous T3 lines were obtained having a phenotype qualitatively equivalent to the line shown here.

GenotypeNo. of Nuclei per TISNo. of TIS
Col-01.00 ± 0.00 a50
sim2.16 ± 0.9850
sim GL2pro:Δ104-1271.08 ± 0.27 a50
sim GL2pro:Δ82-1271.02 ± 0.14 a50
sim GL2pro:Δ66-1271.84 ± 0.7150
sim GL2pro:nls11.04 ± 0.20 a50
sim GL2pro:nls21.02 ± 0.14 a50
sim GL2pro:nls1 nls21.50 ± 0.74 b50