Table 2. Substitution of the T35 residue of SIM with Asp (T35D) can restore function to the SIM T35A mutant

The multicellular trichome phenotype of a complementation line homozygous for a single T-DNA insert for each of the indicated SMRs was assessed by counting the number of DAPI-stained nuclei at each trichome initiation site (TIS) for each genotype. All values followed by the letter a have significantly fewer nuclei per TIS than the sim mutant (P < 1 × 10−6 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). For each transgenic genotype, at least two additional independent homozygous T3 lines were obtained having a phenotype qualitatively equivalent to the line shown here.

GenotypeNo. of Nuclei per TISNo. of TIS
Col-01.02 ± 0.02 a50
sim2.24 ± 3.0050
sim GL2pro:YFP:SIM1.00 ± 0.00 a50
sim GL2pro:YFP:T35A2.16 ± 1.2050
sim GL2pro:YFP:T35D1.08 ± 0.27 a50