Table 1. Breakpoint identification and PCR verification
Pairs of StrainsChromosomeBreakpoint RangeNo. of ReadsInsertion GapGene IdentifierGene NameMutant in Strain 1Mutant in Strain 2
bp
1A4-1D42Unknown–6,876,0171Open endedCre02.g119651Cre02.g119651YesNo
34,539,389–4,539,406618Cre03.g176651MYSM1NoYes
174,322,754–4,322,816663Cre17.g730950FLA10YesNo
2F1-4C216,737,064–6,737,071118Cre01.g048400DZIP1LYesNo
37,296,662–7,296,6981137Cre03.g206950Cre03.g206950YesNo
51,027,021–1,027,042422Cre05.g246553Cre05.g246553YesNo
173,105,419–3,105,4281110Cre17.g721250FAP22NoYes
3F8-3D114,336,001–4,336,1359135Cre01.g029400TRPC3NoYes
27,226,958–7,227,7989841Cre02.g145950Cre02.g145950YesNo
27,290,053–unknown2Open endedCre02.g145500PTK24YesNo
41,568,203–1,568,5288326Cre04.g213400SUB7YesNo
104,519,930–4,520,0534124No predicted geneNo predicted geneYesNo
127,660,573–unknown8Open endedNo predicted geneNo predicted geneYesNo
17Unknown–768,5663Open endedNo predicted geneNo predicted geneNoNo
17768,925–unknown7Open endedNo predicted geneNo predicted geneNoNo
5E3-5F1165,109,304–5,109,339636Cre06.g281050VPS5ANoYes
92,129,462–2,129,485324Cre09.g393551Cre09.g393551YesNo
166,582,926–6,582,93085Cre16.g672200BLD11YesNo
8D6-9H4141,860,469–1,860,4781410Cre14.g620500Cre14.g620500NoYes
166,583,673–6,583,7081236Cre16.g672200BLD11YesNo
165,635,438–5,635,438150Cre16.g679550FAP277NoYes
8C12-9D521,736,814–unknown7Open endedCre02.g086300Cre02.g086300NoYes
65,398,524–5,398,526133Cre06.g283900TMEM45BYesNo
96,286,926–6,286,995970Cre09.g406500Cre09.g406500NoYes
16Unknown–6,571,4441Open endedCre16.g672250MPA13NoNo
6C2-6A1251,676,418–unknown2Open endedCre05.g242850Cre05.g242850NoYes
6Unknown–3,512,4121Open endedCre06.g278107Cre06.g278107NoYes
64,339,867–4,339,9171351Cre06.g278262Cre06.g278262YesNo
12762,534–unknown2Open endedCre12.g495500Cre12.g495500NoYes
6F2-6B10161,141,900–unknown2Open endedCre16.g650200MITC17YesNo
161,266,125–1,266,130156Cre16.g651350ATG11NoYes
7F3-9A962,254,537–2,254,6121976Cre06.g266450Cre06.g266450YesNo
61,938,823–1,938,8491027Cre06.g263650Cre06.g263650YesNo
9Unknown–497,6603Open endedCre09.g404201PHC10NoYes
133,741,836–unknown3Open endedCre13.g589450Cre13.g589450NoYes
173,468,906–3,468,91196No predicted geneNo predicted geneNoYes
1D11-6D11111,722,733–1,722,733210Cre11.g467784SOULYesNo
161,549,854–1,549,855112Cre16.g653450BAR1YesNo
17Unknown–5,178,8173Open endedCre17.g735550Cre17.g735550NoYes
175,244,723–unknown1Open endedCre17.g736000Cre17.g736000NoYes
17Unknown–5,430,3654Open endedCre17.g736800Cre17.g736800YesNo