Table 1. Maternal effects of MIR167A

For each cross group (except for mir167a−/− × Col-0), we genotyped all the germinated seedlings for each (zygote) genotype.

Cross Group ♀ × ♂Cross NumberCentral CellEggSpermEndospermZygoteMaternal SporophyteSeed Phenotype and Number
Col-0 × mir167a+/−1+/++++/+/++/++/+Normal, n = 49
2+/+++/+/−+/−+/+Normal, n = 50
mir167a+/− × Col-03+/++++/+/++/++/−Normal, n = 34
4−/−++/−/−+/−+/−Normal, n = 27
mir167a+/− selfinga5+/++++/+/++/++/−Normal, n = 36
6+/+++/+/−+/−+/−Normal, n = 64b
7−/−++/−/−+/−+/−
8−/−−/−/−−/−+/−Normal, n = 31
mir167a−/− × Col-09−/−++/−/−+/−−/−Abnormal, n = 132
  • a The χ2 value is calculated based on the expected zygote segregation ratio: AA:Aa:aa = 1:2:1. χ2 = Σ (observed value-expected value)2/expected value. χ2 = 0.45, P > 0.5. The segregation ratio is consistent with the typical Mendelian ratio (1:2:1).

  • b In the offspring of the mir167a+/− self cross, since cross 6 cannot be differentiated from cross 7 via genotyping, n shows the total number of seeds of cross 6 and cross 7.