Table 1. Selected protein spots identified from the DIGE two-dimensional gels shown in Figure 3

The mab1/wild type column shows the ratios of mab1-1 protein abundance to wild type (Col), with significant changes shown in the t test column; the experiment was performed with three replicates. Proteins whose spot numbers are indicated in Figure 3, C to E, were identified by matrix-assisted laser-desorption ionization time of flight tandem mass spectrometry. The predicted molecular mass and pI of the match are shown along with the molecular weight search score (P < 0.05, when the score is >37), number of peptides matched to tandem mass spectra, and percentage coverage of the matched sequence.

Spot No.AccessionDescriptionmab1/Wild Typet TestScoreMatched PeptidesCoverage (%)MasspI
1At5g50850MAB17.280.0021188514.3394365.67
2At5g50850MAB135.410.0003290514.3394365.67
3At5g50850MAB1−15.300.0017138311.3394365.67
4At5g50850MAB1−25.150.0015298614.3394365.67
5At1g59900IAR4L−1.630.001118338.5434887.16
6At1g24180IAR4−1.980.0034175412.2437878.03
7At1g03090MCCA2.330.00516412.9787766.31