Enzymes were heterologously expressed in E. coli, purified, and tested with different aldehydes as potential substrates in the presence of NADPH. Enzyme products were extracted with hexane and analyzed using GC-MS. Symbols: −, no activity detected; + activity detected.
| Substrate | Product | PtPAR1 | PtPAR2 | PtAAR1 | PtAAR2 | PtAAR3 |
|---|---|---|---|---|---|---|
| Citronellal | Citronellol | + | + | + | + | + |
| Geranial | Geraniol | + | + | − | − | − |
| Nonanal | Nonanol | + | + | + | − | − |
| Decanal | Decanol | + | + | + | − | + |
| Hexanal | Hexanol | + | + | − | − | − |
| (E)-2-hexenal | (E)-2-hexenol | + | + | − | − | − |
| Benzaldehyde | Benzyl alcohol | + | + | − | − | − |
| 2-phenylacetaldehyde | 2-phenylethanol | + | + | − | − | − |