Table 2. Enzymatic activity of poplar aldehyde reductases

Enzymes were heterologously expressed in E. coli, purified, and tested with different aldehydes as potential substrates in the presence of NADPH. Enzyme products were extracted with hexane and analyzed using GC-MS. Symbols: −, no activity detected; + activity detected.

SubstrateProductPtPAR1PtPAR2PtAAR1PtAAR2PtAAR3
CitronellalCitronellol+++++
GeranialGeraniol++
NonanalNonanol+++
DecanalDecanol++++
HexanalHexanol++
(E)-2-hexenal(E)-2-hexenol++
BenzaldehydeBenzyl alcohol++
2-phenylacetaldehyde2-phenylethanol++