Table 1. Qualitative PCR analysis to verify the presence of the transgene for overexpression of ca1pase

In addition to the experiment described in this manuscript (Experiment 2), a previous experiment was conducted that showed identical results (Experiment 1). Of the 10 plants investigated per line, the transgene was present in all plants in lines OE1 and OE3 (likely homozygous), while it was present in only six to eight plants of lines OE2 and OE4 (heterozygous).

Transgenic LineNo. of Plants Containing the TransgeneZygosity
Experiment 1Experiment 2
Wild type0/100/10Negative control
OE110/1010/10Likely homozygous
OE310/1010/10Likely homozygous