Table 1. Rubisco catalytic properties

Rubisco maximum VC and KC are shown for wild-type tobacco and transplastomic lines expressing β-cyanobacterial carboxysome components from Se: Rubisco LSU (L), Rubisco SSU (S), and CcmM35 (M35). Values represent means ± se (n = 3–5 biological replicates). *, Wild-type values are from Occhialini et al. (2016). Letters denote significant differences (P < 0.05) between transplastomic lines as determined by Tukey’s pairwise comparisons following ANOVA.

LineVCKC
µmol mg−1 min−1µm
Wild type*3.9 ± 0.29.0 ± 0.3
SeLS15.0 ± 0.9 a168 ± 59 a
SeL0.6 ± 0.2 b105 ± 9 a
SeLSM3510.9 ± 0.8 c133 ± 12 a
SeLM352.0 ± 0.3 b110 ± 22 a