Table I. Lignin analyses for the wild type and BdLAC5 (Bd4442) and BdLAC6 (Bd5024) misregulated mutants

Lignin content and structure for extractive-free mature stems were measured by Klason and thioacidolysis methods. Values are means ± sd from individually analyzed plants (n = 3).

Culture and GenotypeLignin ContentRelative Frequency of Lignin-Derived Thioacidolysis Monomers
HGS
%
First culture
 Wild type17.63 ± 0.012.8 ± 0.130.9 ± 0.866.3 ± 0.9
 Bd444215.71 ± 0.04a2.6 ± 0.225.3 ± 0.4a72.2 ± 0.6a
 Bd502417.53 ± 0.142.8 ± 0.133.3 ± 0.363.9 ± 0.3
Second culture
 Wild type16.93 ± 0.573.0 ± 0.230.1 ± 2.566.9 ± 2.7
 Bd444215.60 ± 0.07a1.6 ± 0.2a22.2 ± 3.5a76.3 ± 3.5a
Third culture
 Wild type17.59 ± 0.493.3 ± 0.131.0 ± 1.065.7 ± 1.0
 Bd444216.19 ± 0.20a2.7 ± 0.126.5 ± 0.5a70.8 ± 0.5a
  • a Significantly different from the corresponding wild-type sample (one-way ANOVA, Tukey’s honestly significant difference) at P < 0.05.