Table IV. Saccharification assays of extractive-free mature stem from the wild type and the BdLAC5 (Bd4442) mutant line as compared with the Bd4442 CP3 mutant line complemented with the BdLAC5 wild-type allele (T2 transformants)

Saccharification was evaluated both by the weight loss percentage and by the amount of Glc released from the samples. The data represent means ± sd from biological replicates (two independent experiments performed each on three different samples per line). Letters indicate significant differences analyzed by one-way ANOVA (Tukey’s honestly significant difference, P < 0.05).

GenotypeWeight Loss Percentage Glc
mg g−1
Bd21-3 wild type23.5 ± 1.4 a68.5 ± 3.9 a
Bd4442 homozygous30.5 ± 1.3 b113.6 ± 4.4 d
Bd4442 CP320.6 ± 0.4 c75.3 ± 3.3 a,b