Table II. Effects of zoledronate and fosmidomycin on average (±se) isoprene emission rate, DMADP pool size, dark pool size, rate constant of isoprene synthase reaction, and K_m and V_max of isoprene synthase in vivo in leaves of hybrid aspen

Definition of all characteristics, number of replicates, and statistical comparison of treatments are as in Table I. Two measurement protocols were used. In the case of the first protocol, measurements were started without inhibitors (control), followed by a 40-min treatment with zoledronate and a 30-min treatment with fosmidomycin. In the case of the second protocol, measurements were started without inhibitors (control) and were followed by a 30-min treatment with fosmidomycin and a 40-min treatment with zoledronate.

Treatment	Isoprene Emission Rate	DMADP Pool Size	Dark Pool Size	Initial Slope	K_m	V_max
	nmol m⁻² s⁻¹	nmol m⁻²		s⁻¹	nmol m⁻²	nmol m⁻² s⁻¹
Control	27.2 ± 1.7a	1160 ± 150b	1600 ± 150c	0.0331 ± 0.0022a	2370 ± 110ab	78 ± 7a
Zoledronate (Zld, 40 min)	19.3 ± 1.6b	1458 ± 90a	5900 ± 500a	0.0197 ± 0.0021b	2430 ± 120a	50 ± 5b
Fosmidomycin (Fmd, 30 min)	2.15 ± 0.31d	78 ± 4.5d	852 ± 60d	0.0341 ± 0.0018a	NA	NA
Zld+Fmd (40 +30 min)	10.5 ± 0.9c	615 ± 38c	6270 ± 70a	0.0212 ± 0.0012b	2280 ± 60b	47.1 ± 4.5b
Fmd+Zld (30 +40 min)	1.64 ± 0.23d	35 ± 4.8e	3960 ± 210b	0.0233 ± 0.0031b	NA	NA