Table II. Physicochemical properties of FRET fluorophores
FRET PairDonor Excitation λmaxAcceptor Emission λmaxDonor Quantum YieldAcceptor Absorption CoefficientFörster Distance
DonorAcceptor
nmm−1 cm−1nm
mTRQ2mVenus4305290.9392,2005.7
mEGFPmCherry4886110.672,0005.2
mVenustagRFP5165830.57100,0005.9