Table V. Leaf metabolite profiling of gox3-1 and GOX3-OX1 fed with [13C]l-lactate and [13C]glycolate

Values are presented as means ± se from four individual samples. GOX3-OX1 values in boldface are significantly different from gox3-1 values, and asterisks indicate significant differences between the two feeding experiments according to a two-way ANOVA (Tukey’s test; P < 0.05).

Metabolite[13C]l-Lactate[13C]Glycolate
gox3-1GOX3-OX1gox3-1GOX3-OX1
nmol g−1 fresh wt
Ala197.56 ± 55.52484.37 ± 54.31133.98 ± 5.43325.03 ± 25.40*
Arg150.98 ± 41.24973.13 ± 194.90164.26 ± 47.81584.40 ± 104.12*
Asn9.03 ± 1.73106.96 ± 20.5720.02 ± 3.3666.35 ± 9.48*
Asp20.89 ± 6.30114.99 ± 17.2321.16 ± 3.9451.28 ± 5.84*
Citrate1.99 ± 0.382.83 ± 0.861.27 ± 0.461.57 ± 0.22
Dehydroascorbate107.09 ± 19.56268.32 ± 58.9087.01 ± 43.27124.47 ± 11.05*
Erythritol0.26 ± 0.070.48 ± 0.090.23 ± 0.010.43 ± 0.06
Fru67.14 ± 5.5196.28 ± 14.23185.54 ± 65.59145.43 ± 43.05
Fumarate319.78 ± 46.36689.31 ± 128.78317.89 ± 68.39702.86 ± 233.32
GABA12.94 ± 2.4621.77 ± 5.078.48 ± 2.1513.68 ± 3.47
Glc115.77 ± 16.28280.83 ± 27.89294.58 ± 84.07360.00 ± 81.24
Glu160.07 ± 13.68617.20 ± 104.64172.41 ± 31.58369.52 ± 71.51*
Gln129.55 ± 19.38821.61 ± 56.47186.16 ± 20.05622.72 ± 49.86*
Glycerate2.78 ± 0.505.35 ± 1.354.61 ± 0.666.12 ± 0.86
Gly9.16 ± 2.9472.09 ± 18.2017.49 ± 2.3449.09 ± 10.01
Glycolate3.16 ± 0.975.71 ± 0.6712.58 ± 1.1612.01 ± 0.41*
Inositol106.13 ± 17.52225.21 ± 22.27101.08 ± 16.34179.22 ± 14.54
Ile2.47 ± 0.359.44 ± 1.493.05 ± 0.195.65 ± 0.26*
Lys1.93 ± 0.399.87 ± 1.901.88 ± 0.155.74 ± 0.30*
Malate48.08 ± 9.84262.84 ± 48.5155.74 ± 11.81146.27 ± 4.27*
Maltose10.65 ± 3.6024.25 ± 9.815.91 ± 1.387.13 ± 1.57*
Met1.08 ± 0.233.35 ± 0.280.82 ± 0.182.98 ± 0.44
Phe4.23 ± 1.0810.90 ± 1.005.38 ± 1.548.98 ± 1.01
Pro129.51 ± 45.20923.99 ± 109.5555.36 ± 17.83617.31 ± 197.18
Putrescine4.11 ± 1.1318.24 ± 3.597.04 ± 1.6911.44 ± 1.10*
Pyruvate2.56 ± 0.857.94 ± 1.823.93 ± 1.104.57 ± 0.40
Raffinose58.00 ± 20.4776.91 ± 6.1727.12 ± 5.2144.53 ± 12.40
Ser62.92 ± 18.88212.35 ± 6.2895.66 ± 11.34174.59 ± 14.92
Sorbose30.84 ± 3.7254.26 ± 8.2892.93 ± 34.1674.67 ± 20.88
Spermidine5.22 ± 0.777.26 ± 2.013.14 ± 0.726.43 ± 0.34
Succinate18.63 ± 2.3549.42 ± 8.1911.98 ± 1.7929.29 ± 1.14*
Suc33.01 ± 9.3691.00 ± 14.6727.14 ± 0.5254.15 ± 4.18*
Threonate32.52 ± 5.9294.32 ± 23.9562.08 ± 11.9575.12 ± 13.17
Thr25.68 ± 7.1796.61 ± 8.9225.28 ± 3.6663.12 ± 4.96*
Trp17.97 ± 2.5836.17 ± 6.9720.03 ± 3.8227.79 ± 4.29
Tyr0.73 ± 0.152.02 ± 0.340.94 ± 0.361.40 ± 0.24
Val6.36 ± 1.1022.33 ± 3.455.78 ± 0.4013.71 ± 2.06*