Table I. Characterization of the prosthetic group of GOX1, GOX2, and GOX3

The prosthetic groups of the enzymes were released by boiling and spotted together with FMN, FAD, and riboflavin onto a cellulose 300 TLC plate. The TLCs were run in the dark with the solvent mixtures 5% Na2HPO4 in aqueous solution (A) and 1-butanol:acetic acid:water (4:1:5; B). The sample positions were detected under UV light.

SampleRF Values
TLC ATLC B
FMN0.660.27
FAD0.560.10
Riboflavin0.370.46
GOX1 group0.700.27
GOX2 group0.680.27
GOX3 group0.630.27