Table III. Kinetic constants of GOX1, GOX2, and GOX3

Kinetic constants were determined using the standard reaction mix either of the oxidase assay or the dehydrogenase assay, varying the substrate concentration from 0 to 37.9 mm; 1 to 5 µg of purified enzyme was used. Kinetic data were determined using the nonlinear regression fit to the Michaelis-Menten equation f(x) = ax/b + x (Sigma Plot software). Shown are mean values ± se of three independent experiments.

SampleKmkcatkcat/Km
µmmin−1min−1 mm−1
GOX1
 Glycolate278 ± 13254 ± 2911
l-Lactate2,753 ± 165114 ± 241
GOX2
 Glycolate327 ± 21224 ± 4744
l-Lactate4,905 ± 241116 ± 224
GOX3
 Glycolate144 ± 10104 ± 1725
l-Lactate420 ± 480 ± 2190