Table VI. Redistribution of label (total enrichment) following feeding of leaves with [13C]l-lactate and [13C]glycolate in gox3-1 and GOX3-OX1

Values are presented as means ± se of four individual samples. GOX3-OX1 values in boldface are significantly different from gox3-1 values, and asterisks indicate significant differences between the two feeding experiments according to a two-way ANOVA (Tukey’s test; P < 0.05).

Metabolite[13C]l-Lactate[13C]Glycolate
gox3-1GOX3-OX1gox3-1GOX3-OX1
nmol g−1 fresh wt
Ala40.07 ± 9.3153.89 ± 15.714.53 ± 0.60*6.73 ± 0.56*
Arg1.70 ± 1.316.03 ± 3.760.53 ± 0.536.76 ± 3.80
Asn0.44 ± 0.044.36 ± 1.650.87 ± 0.052.40 ± 0.27
Asp0.24 ± 0.090.55 ± 0.150.34 ± 0.030.74 ± 0.14
Citrate0.03 ± 0.010.02 ± 0.020.02 ± 0.010.01 ± 0.01
Erythritol0.01 ± 0.000.00 ± 0.000.00 ± 0.000.01 ± 0.01
Fru0.42 ± 0.080.62 ± 0.140.94 ± 0.230.98 ± 0.32
Fumarate0.00 ± 0.000.00 ± 0.000.09 ± 0.090.13 ± 0.13
GABA0.48 ± 0.080.45 ± 0.120.11 ± 0.060.11 ± 0.02
Glc2.59 ± 0.356.28 ± 0.567.29 ± 2.23*8.26 ± 1.84
Glu3.92 ± 0.408.44 ± 2.581.23 ± 0.172.24 ± 0.44*
Gln22.52 ± 2.7261.07 ± 7.1350.85 ± 13.8766.65 ± 14.02
Glycerate0.09 ± 0.010.02 ± 0.020.75 ± 0.16*0.83 ± 0.07*
Gly0.04 ± 0.010.11 ± 0.071.50 ± 0.40*1.89 ± 0.27*
Glycolate0.58 ± 0.090.13 ± 0.1310.19 ± 1.06*8.09 ± 0.28*
Inositol0.79 ± 0.142.23 ± 0.240.83 ± 0.131.65 ± 0.06*
Ile0.06 ± 0.010.15 ± 0.020.01 ± 0.00*0.02 ± 0.00*
Lys0.09 ± 0.020.40 ± 0.050.03 ± 0.020.23 ± 0.03*
Malate0.15 ± 0.041.39 ± 0.570.53 ± 0.151.12 ± 0.16
Maltose1.12 ± 0.352.76 ± 1.030.78 ± 0.190.78 ± 0.17*
Met0.00 ± 0.000.01 ± 0.010.02 ± 0.000.07 ± 0.02*
Phe0.07 ± 0.010.28 ± 0.030.09 ± 0.000.18 ± 0.04*
Pro2.77 ± 0.8518.20 ± 2.431.30 ± 0.2410.27 ± 3.70*
Raffinose0.42 ± 0.160.87 ± 0.150.25 ± 0.060.50 ± 0.15
Ser0.61 ± 0.220.76 ± 0.1913.56 ± 2.14*22.89 ± 3.87*
Succinate0.61 ± 0.040.80 ± 0.490.07 ± 0.030.05 ± 0.01
Suc1.31 ± 0.302.89 ± 0.442.30 ± 0.084.27 ± 0.30*
Threonate1.75 ± 0.275.25 ± 1.193.29 ± 0.644.31 ± 0.71
Thr0.36 ± 0.091.26 ± 0.050.39 ± 0.050.95 ± 0.11*
Tyr0.00 ± 0.000.00 ± 0.000.01 ± 0.000.00 ± 0.00
Val1.08 ± 0.153.21 ± 0.470.68 ± 0.051.57 ± 0.23*