Table VII. Relative metabolite levels (to the internal standard ribitol) measured by gas chromatography-mass spectrometry of roots under normoxia and after 8 and 24 h of hypoxia (8 h, −oxygen and 24 h, −oxygen)

Values are presented as means ± se from at least four replicates. Values in boldface indicate significant differences from 0 h for each genotype, and asterisks indicate significant differences from the wild type at the corresponding time point, according to a two-way ANOVA (Tukey’s test; P < 0.05).

Metabolite0 h8 h, −Oxygen24 h, −Oxygen
Wild Typegox3-1Wild Typegox3-1Wild Typegox3-1
Sugars
 Fru87.1 ± 15.5204.7 ± 22.4*190.5 ± 27.9227.7 ± 42.8251.2 ± 37.4299.2 ± 72.8
 Gluconate2.0 ± 0.53.9 ± 0.2*3.0 ± 0.33.7 ± 0.12.5 ± 0.23.5 ± 0.6
 Glc485.2 ± 104.41120.7 ± 134.0*800.0 ± 87.6998.7 ± 203.7890.1 ± 129.01,280.4 ± 237.3
 Maltose207.3 ± 41.8303.7 ± 17.9*301.2 ± 7.1372.0 ± 26.4396.7 ± 11.6299.5 ± 14.4*
 Mannitol9.6 ± 2.98.6 ± 2.57.7 ± 0.37.1 ± 0.817.0 ± 3.012.6 ± 2.0
 Man2.4 ± 0.52.8 ± 0.35.2 ± 0.47.6 ± 1.7*4.3 ± 1.04.4 ± 0.2
 Myoinositol562.7 ± 85.1827.5 ± 45.8*713.4 ± 33.9857.2 ± 69.8966.9 ± 31.3829.2 ± 18.7
 Raffinose7.2 ± 2.66.4 ± 0.42.3 ± 0.232.8 ± 0.38.1 ± 1.810.7 ± 3.9
 Sorbitol30.2 ± 5.945.2 ± 13.320.9 ± 5.313.9 ± 1.464.7 ± 5.948.2 ± 2.2
 Suc11,512.1 ± 1,331.312,855.0 ± 171.213,864.7 ± 1,221.015,837.4 ± 901.416,608.7 ± 1,197.914,536.9 ± 1,623.8
 Xyl6.0 ± 1.111.2 ± 1.5*5.3 ± 0.79.0 ± 1.3*7.4 ± 0.711.1 ± 1.1*
Amino acids
 Ala273.8 ± 35.6398.1 ± 9.0*545.3 ± 9.6678.0 ± 35.3697.4 ± 21.8797.0 ± 204.3
 Asp41.4 ± 7.426.9 ± 2.946.4 ± 2.7354.0 ± 10.7488.3 ± 9.0557.6 ± 15.8
 β-Ala1.2 ± 0.23.4 ± 0.3*2.4 ± 0.12.5 ± 0.333.0 ± 0.13.6 ± 0.4
GABA26.8 ± 7.265.6 ± 6.1*71.9 ± 3.269.7 ± 7.062.8 ± 3.856.2 ± 6.6
 Glu206.2 ± 49.5114.0 ± 25.3485.3 ± 39.7534.6 ± 117.9573.1 ± 94.9368.0 ± 119.6
 Ile29.8 ± 4.932.5 ± 0.751.5 ± 4.068.2 ± 4.85*40.0 ± 2.634.3 ± 3.8
 Leu30.8 ± 4.531.3 ± 0.745.8 ± 3.559.78 ± 5.0*29.9 ± 2.526.9 ± 3.4
 Met9.7 ± 1.04.2 ± 0.626.4 ± 2.520.7 ± 5.024.5 ± 1.715.0 ± 6.8
 Phe5.9 ± 1.41.8 ± 0.739.9 ± 2.89.8 ± 2.1*12.9 ± 1.94.2 ± 1.4*
 Pro6.4 ± 1.08.2 ± 0.56.3 ± 0.210.4 ± 0.9*6.7 ± 1.38.8 ± 1.9
 Shikimate46.5 ± 7.870.8 ± 3.2*46.1 ± 1.351.5 ± 3.774.7 ± 3.083.0 ± 8.6
 Thr94.3 ± 19.199.1 ± 10.891.7 ± 2.5130.2 ± 10.8*137.4 ± 14.6116.3 ± 11.0
 Trp38.1 ± 7.324.1 ± 1.9*34.4 ± 2.415.2 ± 0.7*20.5 ± 1.510.6 ± 0.8*
 Val90.0 ± 14.7103 ± 2.5117.9 ± 6.8159.2 ± 8.4*117.8 ± 6.5106.6 ± 8.4
Photorespiration
 Glycerate8.0 ± 1.09.0 ± 0.37.3 ± 0.38.4 ± 0.410.8 ± 0.211.2 ± 0.7
 Gly36.3 ± 6.856.5 ± 5.4*72.8 ± 3.054.2 ± 5.2*76.8 ± 11.366.7 ± 4.1
 Glycolate220.1 ± 9.9247.7 ± 4.3257.7 ± 18.5240.9 ± 20.5261.6 ± 33.6259.5 ± 26.9
 Ser74.9 ± 15.663.7 ± 6.2100.9 ± 3.6135.6 ± 10.8105.2 ± 16.090.8 ± 19.5
Tricarboxylic acid
 (Iso)citrate435.7 ± 61.3663.6 ± 25.8*495.0 ± 17.2515.7 ± 20.5633.4 ± 14.7660.5 ± 44.97
 α-Ketoglutarate5.9 ± 1.97.1 ± 0.89.2 ± 0.813.2 ± 2.57.4 ± 0.97.3 ± 1.9
 2-Hydroxyglutarate3.1 ± 0.93.7 ± 0.84.6 ± 0.56.37 ± 1.17.0 ± 0.94.9 ± 0.1*
 Fumarate21.7 ± 2.538.6 ± 0.6*24.6 ± 0.426.2 ± 0.924.5 ± 1.032.2 ± 2.6*
 Malate259.4 ± 34.1354.7 ± 11.7*282.9 ± 5.6364.4 ± 20.8*266.0 ± 2.3339.5 ± 37.6*
 Succinate39.0 ± 4.345.8 ± 1.253.1 ± 1.449.2 ± 2.951.2 ± 3.849.4 ± 3.4
Others
 Glycerol281.0 ± 32.2320.3 ± 33.4309.8 ± 10.0331.5 ± 18.1339.6 ± 13.8307.8 ± 15.7
 Hydroxy butyrate13.16 ± 1.367.6 ± 0.415.9 ± 0.810.7 ± 3.713.6 ± 2.66.3 ± 1.8*
 Lactate917.1 ± 76.1691.0 ± 175.3993.9 ± 171.3610.1 ± 105.8*687.8 ± 133.7537.5 ± 111.7
 Malate2.0 ± 0.61.5 ± 0.23.6 ± 0.51.9 ± 0.37*2.7 ± 0.51.6 ± 0.1*
 Malonate0.6 ± 0.10.6 ± 0.10.8 ± 0.10.8 ± 0.00.8 ± 0.20.8 ± 0.1
 Oxalate60.68 ± 9.070.7 ± 5.782.8 ± 8.778.0 ± 8.38173.4 ± 16.5145.3 ± 6.3*
 Quinic acid3.98 ± 1.22.8 ± 0.33.1 ± 0.23.1 ± 0.53.8 ± 0.62.8 ± 0.3