Table II. Glycosyl linkages in total mucilage extracts

Mean relative abundance (mol %) of each linkage ± sd of three or two (irx14-2) biological replicates.

LinkageWild Typemuci21-1irx14-2
Rha
 t-Rha1.68 ± 1.381.12 ± 0.243.74 ± 2.38
 2-Rha31.89 ± 0.5426.37 ± 4.1430.62 ± 0.17
 2,3-Rha1.40 ± 0.572.76 ± 1.251.46 ± 0.10
 2,4-Rha0.96 ± 0.713.35 ± 1.780.99 ± 0.66
Ara
 t-Ara0.15 ± 0.080.05 ± 0.030.13 ± 0.00
 5-Ara0.09 ± 0.010.46 ± 0.03a0.15 ± 0.10
 3-Ara0.62 ± 0.090.40 ± 0.04a0.62 ± 0.15
Gal
 t-Gal0.42 ± 0.170.28 ± 0.050.22 ± 0.06
 2-Gal0.17 ± 0.010.13 ± 0.02a0.13 ± 0.08
 4-Gal0.20 ± 0.040.15 ± 0.020.12 ± 0.00
 6-Gal0.09 ± 0.010.10 ± 0.030.18 ± 0.03a
 2,4-Gal0.11 ± 0.090.12 ± 0.010.09 ± 0.02
 4,6-Gal0.18 ± 0.040.13 ± 0.020.18 ± 0.17
 3,6-Gal0.43 ± 0.150.48 ± 0.030.39 ± 0.18
Glc
 t-Glc0.03 ± 0.000.09 ± 0.01a0.03 ± 0.00
 4-Glc0.79 ± 0.030.71 ± 0.110.69 ± 0.11
 3,4-Glc0.03 ± 0.010.03 ± 0.010.05 ± 0.00
 4,6-Glc0.09 ± 0.020.13 ± 0.010.14 ± 0.05
Xyl
t-Xyl0.52 ± 0.080.15 ± 0.0a0.25 ± 0.03a
 4-Xyl1.83 ± 0.021.18 ± 0.10a0.10 ± 0.04a
 2,4-Xyl0.73 ± 0.190.09 ± 0.02a0.00 ± 0.00a
Man
 4-Man0.24 ± 0.020.23 ± 0.060.22 ± 0.11
 4,6-Man0.56 ± 0.030.54 ± 0.080.46 ± 0.02a
GalA
 t-GalA0.63 ± 0.240.40 ± 0.080.71 ± 0.47
 4-GalA54.22 ± 1.6054.31 ± 2.1556.37 ± 1.23
 2,4-GalA0.54 ± 0.141.07 ± 0.340.51 ± 0.06
 4,6-GalA1.32 ± 0.875.09 ± 2.811.36 ± 1.18
  • a Significant difference (Student’s t test, P < 0.05) from the wild type.