Table II. Summary of proteins and phosphopeptides identified from soluble leaf extracts by 2D CN-PAGE and MS/MS analysis
Spot Identifier and Change in pp2a-b′γ aFull NameGeneScore/CoveragePhosphopeptidesb
Defense and stress
 TGG1 ↑Thioglucoside glucohydrolase 1AT5G260002,732/39%R.GYALG(p)TDAPGR.CK.GFIFGVAS(p)SAYQVEGGR.GR.EYVGDRLPEF(p)SETEAALVK.GK.EKYE(p)TNPALYGELAK.G
 CA1 ↑Carbonic anhydrase1AT3G015001,224/27%
 AtGSTF2 ↑Glutathione S-transferase F2AT4G025201,028/58%
 CSD2 ↑Copper/zinc superoxide dismutaseAT2G28190229/32%
Amino acid metabolism and Met cycle
 OLD3 ↑Onset of leaf death 3/O-acetyl-Ser(thiol)lyaseAT4G14880688/40%
 SAHH1 ↑S-Adenosyl-l-homocysteine hydrolase 1AT4G139401,375/39%R.LVGV(p)SEETTTGVK.RR.I(p)TIKPQTDR.Wc
 SAHH2 ↑S-Adenosyl-l-homocysteine hydrolase 2AT3G23810265/18%
 ADK1 ↑Adenosine kinase 1AT3G09820484/39%
 ADK2 ↑Adenosine kinase 2AT5G03300482/35%
 GS2 ↓Gln synthetase 2AT5G35630694/24%R.(p)TIEKPVEDPSELPK.W
 GS1.2Gln synthetase 1;2AT1G66200143/10%
 AtGLN1;1 ↑Gln synthetase 1;1AT5G37600262/24%
 AGT1Ala-glyoxylate aminotransferaseAT2G13360499/25%K.(p)TLLEDVKK.IK.L(p)SQDENHTIK.A
Carbon metabolism
 TKL ↑TransketolaseAT3G60750902/30%K.(p)TVTDKPTLIK.VK.(p)SIITGELPAGWEK.AK.AN(p)SYSVHGAALGEK.ER.HTPEGA(p)TLESDWSAK.F
 FBAFru bisphosphate aldolaseAT4G38970582/30%K.Y(p)TGEGESEEAK.ER.A(p)TPEQVAAYTLK.LK.DRA(p)TPEQVAAYTLK.LK.RLDSIGLEN(p)TEANR.Q
 MDHMalate dehydrogenaseAT1G04410774
 FBPFru-1,6-bisphosphataseAT1G43670681/46%
 PRK ↑PhosphoribulokinaseAT1G320601,731/28%K.FYGEV(p)TQQMLK.H
 IDHCytosolic NADP+-dependent isocitrate dehydrogenaseAT1G65930711/17%
Other
 CA2Carbonic anhydrase 2AT5G14740642/50%
 CRT1Calreticulin 1AT1G56340587/33%
 Cpn60α ↑Chaperonin-60αAT2G28000785/31%
  • a The spot identifications are as presented in Figure 4. Protein spots showing higher or lower intensity as compared with the wild type are indicated by arrows.

  • b For identification of phosphopeptides, Mascot analysis of MS/MS spectra was conducted by restricting searches for the Arabidopsis database and allowing phosphorylation of Thr and Ser residues as possible modifications.

  • c This peptide was detected with a score below the statistical limit for reliable identification.