Table I.

Identity of amino acid sequences of Arabidopsis P450 reductases to those of the reductases from various species

SpeciesAR1AR2Accession No.
identity %
A. thaliana(AR1)63This study
A. thaliana(AR2)63This study
H. tuberosus (HTR1)6775Z26250
H. tuberosus(HTR2)-a 6570Z26251
V. sativa 74 67 Z26252
P. aureus 74 67 This study
C. roseus 63 73 X69791
Homo sapiens 41 40 S90469
Rattus norvegicus 40 41 M12516
Drosophila melanogaster 41 42 X93090
Caenorhabditis elegans 37 39 U21322
Saccharomyces cerevisiae 37 37 D13788
Aspergillus niger 38 38 Z26938
Bacillus megaterium -b 3433J04832
  • F0-a HTR2 is a partial cDNA clone lacking the N-terminal region (Fig. 2).

  • F0-b In B. megaterium a gene encodes P450-BM-3, which consists of two domains corresponding to Cyt P450 and P450 reductase (Ruettinger et al., 1989), and, therefore, the region coding for the P450 reductase domain was used for the homology analysis.