Table III.

The reconstituted CYP73A5 activity

Assay ConditionCinnamate 4-Hydroxylase Activity2-a
nmol min−1 nmol−1P450
 AR163.6  ± 4.3
 AR270.2  ± 5.6
Without NADPHn.d.2-c
Without P450 reductasen.d.
Without CYP73A5n.d.
  • F2-a Reactions were carried out in the complete reaction mixture at 30°C and were initiated by addition of NADPH. Values are means ± sd of three separate determinations.

  • F2-b Complete reaction mixture contained 50 mm potassium phosphate (pH 7.25), 5 nm recombinant CYP73A5, 0.1 unit/mL recombinant P450 reductase (AR1 or AR2), 0.01% (w/v) sodium cholate, 10 μg/mL dilauroylphosphatidylcholine, 0.1 mm NADPH, and 0.4 mm trans-cinnamic acid.

  • F2-c n.d., Not detected.