The reconstituted CYP73A5 activity
| Assay Condition | Cinnamate 4-Hydroxylase Activity2-a |
|---|---|
| nmol min−1 nmol−1P450 | |
| Complete2-b | |
| AR1 | 63.6 ± 4.3 |
| AR2 | 70.2 ± 5.6 |
| Without NADPH | n.d.2-c |
| Without P450 reductase | n.d. |
| Without CYP73A5 | n.d. |
↵F2-a Reactions were carried out in the complete reaction mixture at 30°C and were initiated by addition of NADPH. Values are means ± sd of three separate determinations.
↵F2-b Complete reaction mixture contained 50 mm potassium phosphate (pH 7.25), 5 nm recombinant CYP73A5, 0.1 unit/mL recombinant P450 reductase (AR1 or AR2), 0.01% (w/v) sodium cholate, 10 μg/mL dilauroylphosphatidylcholine, 0.1 mm NADPH, and 0.4 mm trans-cinnamic acid.
↵F2-c n.d., Not detected.