AO activity of wild-type and surI mutant seedlings of Arabidopsis using IAld and IAAld as the substrates
| Substrate | Activity | |
|---|---|---|
| Wild type | sur1 | |
| pmol product min−1 mg−1 protein | ||
| IAld | 54.5 | 186.9 |
| IAAld | 22.2 | 119.5 |
The activity was assayed by determining the amounts of ICA and IAA formed during the reaction, as described in Methods. Two independent experiments each with two assays were carried out and one typical result is presented here.