Table II.

Influence of staurosporine on the accumulation of [14C]2,4-D and [3H]NAA

ComponentAccumulation Ratio after 30 s
Control cellsStaurosporine-treated cells
[14C]2,4-D
 Total accumulation0.78  ± 0.010.76  ± 0.02
 Nonsaturable component0.35  ± 0.010.36  ± 0.01
 Saturable component0.40  ± 0.010.39  ± 0.03
[3H]NAA
 Total accumulation0.63  ± 0.030.86  ± 0.04
 Nonsaturable component0.94  ± 0.100.96  ± 0.05
 Saturable component−0.31  ± 0.081-a −0.10  ± 0.021-a

Accumulation of [14C]2,4-D (93–117 nm external concentration) and [3H]NAA (25–29 nm external concentration) was monitored over 30 s in suspension-cultured tobacco cells that had been exposed to 1 μm staurosporine or DMSO (control cells) for 30 min. Experimental procedure and presentation of results are the same as given in Table I. Data, expressed as accumulation ratios, are means ± se of values obtained in two ([14C]2,4-D accumulation) or five ([3H]NAA accumulation) independent experiments, each with three replicates.

    • F1-a Negative values correspond to auxin efflux.