Table I.

Cytokinins detected in the leaf tissue of Cu-ipt tobacco lines ID8 and ID9 under noninductive conditions

CytokininID8ID9
pmol g−1fresh wt
ZRnd-a 134.4
DZRTrace-b Trace
iPATrace9.6
ZOGTrace96.4
ZROGnd46.9
ZNTTrace28.0
TotalTrace315.3

Values have been corrected for losses during purification and for differential cross-reactivity of the antibodies. The detection limit for ZR with clone 16 was 0.6 pmol and for iPA with clone 12 it was 0.8 pmol.

    • F0-a nd, Not detected.

    • F0-b Trace, Quantities detected were below the limit for accurate quantification of 1 pmol g−1 fresh weight.