Cytokinins detected in the leaf tissue of Cu-ipt tobacco lines ID8 and ID9 under noninductive conditions
| Cytokinin | ID8 | ID9 |
|---|---|---|
| pmol g−1fresh wt | ||
| ZR | nd-a | 134.4 |
| DZR | Trace-b | Trace |
| iPA | Trace | 9.6 |
| ZOG | Trace | 96.4 |
| ZROG | nd | 46.9 |
| ZNT | Trace | 28.0 |
| Total | Trace | 315.3 |
Values have been corrected for losses during purification and for differential cross-reactivity of the antibodies. The detection limit for ZR with clone 16 was 0.6 pmol and for iPA with clone 12 it was 0.8 pmol.