Table II.

Comparison of the cytokinins detected in the leaf tissue of Cu-ipt tobacco line ID13 following in vitro treatment with water (−CuSO4) or Cu (+CuSO4).

Cytokinin−CuSO4+CuSO4
pmol g−1 fresh wt
Znd1-a 27.5
DZRnd52.2
iPTrace1-b 40.5
ZOGnd11.8
TotalTrace132.0

Values have been corrected for losses during purification and for differential cross-reactivity of the antibodies. The detection limit for ZR with clone 16 was 0.6 pmol and for iPA with clone 12 it was 0.8 pmol.

    • F1-a nd, Not detected.

    • F1-b Trace, Quantities detected were below the limit for accurate quantification of 1 pmol g−1 fresh weight.