Table I.

Summary of antibody localization patterns of A. longipes extracellular adhesive structures and affinities for highly sulfated and low-sulfate FGGs separated by anion-exchange column chromatography

Adhesive Structure/PolymerAntibodies
Shaft outer layers++++
Shaft core+
Central ribbon+
Pad and collar++++++
Between cells+++
Highly sulfated FGG++++/−+++
Low-sulfate FGG++++/−++++++

Antibodies were detected by secondary anti-mouse polyvalent IgG, IgM and IgA conjugated with FITC. −, Affinity below detectable limits; +/−, low affinity; +, high affinity; and ++, very high affinity.