Table II.

Effect of the ttg mutation and 35S::R transgene on GUS activity in roots of GL2::GUS lines containing GL2-promoter deletions

GL2::GUSTransgene1-aGUS Expression1-bProportion of Wild-Type GUS Activity1-c
ttg-135S::Rttg-135S::R
4-kb GL2promoter+1-d +0.29  ± 0.080.78  ± 0.24
ΔHd++0.19  ± 0.050.61  ± 0.20
ΔRI++0.21  ± 0.110.47  ± 0.23
ΔRV++0.24  ± 0.130.64  ± 0.13
ΔMHp1-e
ΔMX++0.23  ± 0.110.94  ± 0.23
ΔMR
  • F1-a GL2::GUS transgenes defined in Figure 1.

  • F1-b GUS expression in roots, as assessed by histochemical staining.

  • F1-c GUS activity per milligram of protein in the mutant relative to the wild type within a common pool of F2 seedlings containing the indicated GL2promoter::GUS fusion. Values are means ± sd.

  • F1-d +, Typical qualitative pattern of GUS expression detected.

  • F1-e −, No GUS expression detected.