Table IV.

Conservation of the sugar-phosphate-binding motif in sugar nucleotide PPases

EnzymeSourceSugar-Phosphate-Binding Motif3-aRef.
ADPGlc PPaseE. coliIIEFVEKPAN Hill et al. (1991); Preiss and Sivak (1996)
PMI/GMPPseudomonas aeruginosaVQS*****DE May et al. (1994)
PMI/GMPXanthomonas campestrisVER*****LA Köplin et al. (1992)
GDPMan PPaseE. coliRT*****NL Marolda and Valvano (1993)
GDPMan PPaseSalmonella typhimuriumVAE*****DI Jiang et al. (1991)
UDPGlc PPaseE. coliPMVG****KA Hossain et al. (1994)
UDPGlc PPaseBacillus subtilisVKN*****PK Varnó et al. (1993)
UDPGlc PPaseSolanum tuberosum L.TLKI***Y** Katsube et al. (1990)
UDPGlc PPaseDictyostelium discoideumETNK*I**YK Ragheb and Dottin, 1987)
CDPGlc PPaseYersinia pseudotuberculosisVRS*K***KG Thorson et al. (1994)
  • F3-a Lys-195 in E. coli ADPGlc PPase and Lys-175 in P. aeruginosa PMI/GMP, which were shown to bind to Glc-1-P and Man-1-P, respectively, are underlined. Asterisks signify the same amino acid as in the E. coli ADPGlc PPase sequence.