Table I.

Purification of the chicory germacrene A synthase from 75 mL of a 100,000g supernatant (≈34 g of root material)

Purification StepProteinCyclase ActivitySpecific ActivityRecoveryPurification Factor
mgnmol h−1 nmol h−1 mg−1 %
Crude extract (pH 7.0)34.2107331.31001.0
DEAE3.9532181.3302.6
Green 50.4533875131.524
Mono-Q0.033206624219.2201