Table I.

Rop isogene-specific primers for RT-PCR

GenesSense PrimersAntisense PrimersExpected cDNA Length
bp
Rop1At5′-GAAATTAATAAACTTTGAGGGG-3′ (−24 to −3)5′-AGAGATTTCCAATCATCATAG-3′ (+615 to +595)639
Rop2At5′-GCGGCAGAGATGGCGTCAAGG-3′ (−9 to +11)5′-CTTATCACAAGAACGCGCAACG-3′ (+592 to 571)601
Rop3At5′-TACGTAGCTCCATTTCTGGTGGAG-3′ (−41 to −18)5′-CCACAATCCAAGATTGACAGT-3′ (+177 to 157)218
Rop4At5′-CATTATTATCTCTCATCGATTTGG-3′ (−184 to −161)Same as above361-a
Rop5At5′-GTGACATATTTTGGCTCGTCG-3′ (−38 to −18)Same as above215-a
Rop6At5′-CGTCCGTGAGGATGAGTAGT-3′ (−172 to −153)Same as above349
  • F0-a Expected genomic DNA lengths for Rop4At and Rop5At are approximately 800 and 335 bp, respectively.