Table VI.

Lipid peroxidation, H2O2, ascorbate, and glutathione content in peroxisomes isolated from young and dark-induced senescent pea leaves

SenescenceMDAH2O2ASCDHAASC/DHAGSHGSSGGSH/GSSG
dnmol mL−1 nmol mg−1proteinnmol mg−1 protein
 03.6  ± 0.32.9  ± 0.33.7  ± 0.75.4  ± 0.90.684.1  ± 0.80.15  ± 0.0327.3
1117.6  ± 1.95-a 17.3  ± 1.45-b 4.9  ± 0.95-c 7.2  ± 1.15-c 0.6833.6  ± 9.65-a 51.8  ± 10.25-b 0.64

Antioxidants were extracted and determined as described in Methods. For each antioxidant, peroxisomes obtained from two density gradients were combined and processed. Values are expressed as the means ± se of at least six (MDA and H2O2) and four (ascorbate and glutathione) different experiments.

    • F5-a Significantly different from control at P < 0.01.

    • F5-b Significantly different from control at P < 0.001.

    • F5-c Not significantly different from control.