Table III.

High-CO2-requiring strains and Rubisco mutants of C. reinhardtii

Strain or ConstructMutant PhenotypeProcess DisruptedExplanation
Chloroplast rbcLmutations2-a Lack of pyrenoidRubisco packagingLoss of Rubisco; no pyrenoids formed
pmp mutant2-b No HCO3 accumulationHCO3 accumulationPossible loss of transporter
ca-1 alleles2-c Loss of chloroplastic CACO2generationHCO3 not converted to CO2 in chloroplast
cia-5 2-d Poor growth on low CO2 Regulatory mutantsDoes not adjust to low-CO2 conditions