Table I.

Cell death and DNA fragmentation induced by hydrolytic enzymes

HydrolaseConcentration (w/v)DeadTUNEL
%
Control252.7
Macerozyme1404.8
0.1272.5
Pectinase1400.5
0.1393.4
Cellulase1580
0.1371.5
Proteinase K1480
0.1170
Protease XIV1310
0.1221.1
Protease XXIV1540
0.1300
Protease XVII-B1210
0.1350
Papain1613.2
0.1191.1
Chymotrypsin1280
0.1220
Trypsin19621.9
0.1556.0

Cells were treated with the indicated concentration of each hydrolase 67 h after isolation and scored for the percentage of dead cells 6 h later. At least 200 cells were scored for each treatment.